Laser Speckle Contrast Imaging

Laser speckle contrast imaging is an optical imaging technique that can be used to visualise tissue perfusion in real time.  The imaging technique has a high spatial and temporal resolution and can be used to image dynamic changes in cortical cerebral perfusion following disease/brain damage (i.e stroke), neurovascular responses to functional activation (i.e forepaw stimulation) or the effect of administration of pharmacological agents.  Further technical details of the system can be obtained here:

Below is a representative example showing cortical perfusion images at multiple time points from a sham operated rat and a rat that has had a permanent occlusion of the middle cerebral artery (pMCAO).